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one-color microarray-based gene expression analysis  (Agilent technologies)


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    Agilent technologies one-color microarray-based gene expression analysis
    One Color Microarray Based Gene Expression Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+gene+expression+analysis/pm39074525-318-4-1
    Average 90 stars, based on 1 article reviews
    one-color microarray-based gene expression analysis - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Gene Expression:

    Article Title: Analysis of Genes Related to Invadopodia Formation and CTTN in Oral Squamous Cell Carcinoma-A Systematic Gene Expression Analysis.
    Article Snippet: For microarray analysis, we used the Agilent Array platform employing the manufacturer’s standard protocols for sample preparation and microarray hybridization. .. Gene expression analysis was performed with the Whole Human Gene Expression Microarray (4 × 44K; GPL4133), arrays were scanted with the Agilent G2505B Microarray Scanner and feature extraction was performed with Feature Extraction software version 9.5 (all Agilent Technologies, 76337 Waldbronn, Germany). .. Data files from mRNA microarrays were analyzed by GeneSpring GX 7.3.1 according to the manufacturer’s protocol (Agilent Technologies, 76337 Waldbronn, Germany).

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
    Article Snippet: .. In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software. ..

    Article Title: CAR requires Gadd45β to promote phenobarbital-induced mouse liver tumors in early stage
    Article Snippet: RNAs were purified from mouse livers using Trizol reagent (Thermo Fisher Scientific) and the RNeasy mini kit (QIAGEN, Valencia, CA, USA). .. Gene expression analysis was conducted using Agilent Whole Mouse Genome 4 × 44 multiplex format oligo arrays (014868) (Agilent Technologies, Palo Alto, CA, USA) following the Agilent 1-color microarray-based gene expression analysis protocol. ..

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells
    Article Snippet: For microarray analysis, RNA quality was assessed using a Bioanalyzer (Agilent) and then quantified using a Biospecnano instrument (Shimadzu, Kyoto, Japan). .. Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8x60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949) adapted for small amounts of total RNA (100 ng total RNA per reaction). ..

    Article Title: Adipocyte Glucocorticoid Receptor Inhibits Immune Regulatory Genes to Maintain Immune Cell Homeostasis in Adipose Tissue.
    Article Snippet: Glucocorticoids acting via the glucocorticoid receptors (GR) are key regulators of metabolism and the stress response.. However, uncontrolled or excessive GR signaling adversely affects adipose tissue, including endocrine, immune, and metabolic functions.. Inflammation of the adipose tissue promotes systemic metabolic dysfunction; however, the molecular mechanisms underlying the role of adipocyte GR in regulating genes associated with adipose tissue inflammation are poorly understood.

    Microarray:

    Article Title: Analysis of Genes Related to Invadopodia Formation and CTTN in Oral Squamous Cell Carcinoma-A Systematic Gene Expression Analysis.
    Article Snippet: For microarray analysis, we used the Agilent Array platform employing the manufacturer’s standard protocols for sample preparation and microarray hybridization. .. Gene expression analysis was performed with the Whole Human Gene Expression Microarray (4 × 44K; GPL4133), arrays were scanted with the Agilent G2505B Microarray Scanner and feature extraction was performed with Feature Extraction software version 9.5 (all Agilent Technologies, 76337 Waldbronn, Germany). .. Data files from mRNA microarrays were analyzed by GeneSpring GX 7.3.1 according to the manufacturer’s protocol (Agilent Technologies, 76337 Waldbronn, Germany).

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
    Article Snippet: .. In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software. ..

    Article Title: CAR requires Gadd45β to promote phenobarbital-induced mouse liver tumors in early stage
    Article Snippet: RNAs were purified from mouse livers using Trizol reagent (Thermo Fisher Scientific) and the RNeasy mini kit (QIAGEN, Valencia, CA, USA). .. Gene expression analysis was conducted using Agilent Whole Mouse Genome 4 × 44 multiplex format oligo arrays (014868) (Agilent Technologies, Palo Alto, CA, USA) following the Agilent 1-color microarray-based gene expression analysis protocol. ..

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells
    Article Snippet: For microarray analysis, RNA quality was assessed using a Bioanalyzer (Agilent) and then quantified using a Biospecnano instrument (Shimadzu, Kyoto, Japan). .. Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8x60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949) adapted for small amounts of total RNA (100 ng total RNA per reaction). ..

    Article Title: Adipocyte Glucocorticoid Receptor Inhibits Immune Regulatory Genes to Maintain Immune Cell Homeostasis in Adipose Tissue.
    Article Snippet: Glucocorticoids acting via the glucocorticoid receptors (GR) are key regulators of metabolism and the stress response.. However, uncontrolled or excessive GR signaling adversely affects adipose tissue, including endocrine, immune, and metabolic functions.. Inflammation of the adipose tissue promotes systemic metabolic dysfunction; however, the molecular mechanisms underlying the role of adipocyte GR in regulating genes associated with adipose tissue inflammation are poorly understood.

    Extraction:

    Article Title: Analysis of Genes Related to Invadopodia Formation and CTTN in Oral Squamous Cell Carcinoma-A Systematic Gene Expression Analysis.
    Article Snippet: For microarray analysis, we used the Agilent Array platform employing the manufacturer’s standard protocols for sample preparation and microarray hybridization. .. Gene expression analysis was performed with the Whole Human Gene Expression Microarray (4 × 44K; GPL4133), arrays were scanted with the Agilent G2505B Microarray Scanner and feature extraction was performed with Feature Extraction software version 9.5 (all Agilent Technologies, 76337 Waldbronn, Germany). .. Data files from mRNA microarrays were analyzed by GeneSpring GX 7.3.1 according to the manufacturer’s protocol (Agilent Technologies, 76337 Waldbronn, Germany).

    Software:

    Article Title: Analysis of Genes Related to Invadopodia Formation and CTTN in Oral Squamous Cell Carcinoma-A Systematic Gene Expression Analysis.
    Article Snippet: For microarray analysis, we used the Agilent Array platform employing the manufacturer’s standard protocols for sample preparation and microarray hybridization. .. Gene expression analysis was performed with the Whole Human Gene Expression Microarray (4 × 44K; GPL4133), arrays were scanted with the Agilent G2505B Microarray Scanner and feature extraction was performed with Feature Extraction software version 9.5 (all Agilent Technologies, 76337 Waldbronn, Germany). .. Data files from mRNA microarrays were analyzed by GeneSpring GX 7.3.1 according to the manufacturer’s protocol (Agilent Technologies, 76337 Waldbronn, Germany).

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
    Article Snippet: .. In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software. ..

    Purification:

    Article Title: Possible Involvement of Long Non-Coding RNAs GNAS-AS1 and MIR205HG in the Modulation of 5-Fluorouracil Chemosensitivity in Colon Cancer Cells through Increased Extracellular Release of Exosomes
    Article Snippet: .. In this study, purified total RNAs of both the SW480/DR and SW480/DS cells were analyzed using Agilent SurePrint G3 Human Gene Expression v3 8x60k format cDNA microarray and processed in the Agilent GeneSpring GX v14.9.1 software. ..

    other:

    Article Title: ΔNp63 silencing, DNA methylation shifts, and epithelial-mesenchymal transition resulted from TAp63 genome editing in squamous cell carcinoma.
    Article Snippet: Gene expression profiling with microarray Agilent expression array analysis (SurePrint G3 Human GE v3 8 × 60 K Microarray, design ID 072363) was performed.

    Article Title: Chronic oxidative stress adaptation in head and neck cancer cells generates slow-cyclers with decreased tumour growth in vivo.
    Article Snippet: Prior gene expression analysis using a single-colour microarray kit (Agilent Technologies, Germany), RNA concentrations of each sample were determined using a spectrophotometer (NanoDrop 2000C; Thermo Fisher Scientific).

    Article Title: A novel selective NLRP3 inhibitor shows disease-modifying potential in animal models of Parkinson's disease.
    Article Snippet: Pathological activation of the Nod-like receptor family pyrin domain containing protein 3 (NLRP3) inflammasome signaling underlies many autoimmune and neuroinflammatory conditions.. Here we report that, a rationally designed, novel, orally active, selective NLRP3

    Multiplex Assay:

    Article Title: CAR requires Gadd45β to promote phenobarbital-induced mouse liver tumors in early stage
    Article Snippet: RNAs were purified from mouse livers using Trizol reagent (Thermo Fisher Scientific) and the RNeasy mini kit (QIAGEN, Valencia, CA, USA). .. Gene expression analysis was conducted using Agilent Whole Mouse Genome 4 × 44 multiplex format oligo arrays (014868) (Agilent Technologies, Palo Alto, CA, USA) following the Agilent 1-color microarray-based gene expression analysis protocol. ..

    Article Title: Adipocyte Glucocorticoid Receptor Inhibits Immune Regulatory Genes to Maintain Immune Cell Homeostasis in Adipose Tissue.
    Article Snippet: Glucocorticoids acting via the glucocorticoid receptors (GR) are key regulators of metabolism and the stress response.. However, uncontrolled or excessive GR signaling adversely affects adipose tissue, including endocrine, immune, and metabolic functions.. Inflammation of the adipose tissue promotes systemic metabolic dysfunction; however, the molecular mechanisms underlying the role of adipocyte GR in regulating genes associated with adipose tissue inflammation are poorly understood.

    Labeling:

    Article Title: DiPRO1 distinctly reprograms muscle and mesenchymal cancer cells
    Article Snippet: For microarray analysis, RNA quality was assessed using a Bioanalyzer (Agilent) and then quantified using a Biospecnano instrument (Shimadzu, Kyoto, Japan). .. Gene expression analysis was performed using an Agilent® SurePrint G3 Human GE 8x60K Microarray (Agilent Technologies, Santa Clara, CA, USA) using an Agilent Single Color Labeling Kit (Low Input Quick Amp Labeling Kit 034949) adapted for small amounts of total RNA (100 ng total RNA per reaction). ..



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    Agilent technologies microarray-based gene expression analysis guide
    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by <t>microarray</t> analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.
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    Agilent technologies two-color microarray-based gene expression analysis microarrays
    Summary of apoptosis pathways in human and Oncopig cells. Summarized apoptotic pathways altered by gemcitabine treatment in Oncopig BCCL and 5637 cells evaluated by <t>microarray.</t> There were multiple possible cellular pathways involved in apoptosis induction, such as: TP53INP1/P53/BCL/BAX/CASPASE mitochondrial intrinsical pathway; TP53INP1/TP73 activation; FAS/RB1/GADD45B/CASPASE pathway and cell rounding up by RND1 and/or ZMYM6.
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    Image Search Results


    NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Journal: Journal of Experimental Botany

    Article Title: NAC61 regulates late- and post-ripening osmotic, oxidative, and biotic stress responses in grapevine

    doi: 10.1093/jxb/erad507

    Figure Lengend Snippet: NAC61 expression analysis. (A) NAC61 expression behavior in grapevine organs throughout development (bar plot) and compared in the heatmap (logarithmic value) with that of NAC60 and NAC33. The data were retrieved from the atlas transcriptomic dataset of cv. ‘Corvina’ ( Fasoli et al ., 2012 ). Each value represents the mean ±SD of three biological replicates. (B) Correlation between NAC61 expression level and sugar content in grape berries sampled from fruit set to maturity in cv. ‘Cabernet Sauvignon’ and cv. ‘Pinot noir’ ( Fasoli et al ., 2018 ). The black line represents the trend of the averaged values of the two varieties. The R 2 values shown correspond to the fitting of different polynomial regressions to each corresponding group of samples (orange for cv. ‘Cabernet Sauvignon’ samples, blue for cv. ‘Pinot noir’ samples, and black for the entire set of samples). (C) Correlation between NAC61 expression level and sugar content in grape berries sampled during post-harvest dehydration in six different varieties ( Zenoni et al ., 2016 ). (D) Correlation between NAC61 expression level and berry weight loss in cv. ‘Corvina’ berries sampled during traditional long and forced short post-harvest dehydration processes ( Zenoni et al ., 2020 ). Expression values were determined by microarray analysis and each value represents the mean ±SD from three biological replicates. (E) NAC61 GCNs based on berry, leaf, and tissue-independent (TI) datasets. Left, Venn diagram showing exclusive and shared genes based on the three datasets; right, three-dimensional plot of co-expressed genes in which NAC, WRKY, and ZIP family members already described as having involvement in berry ripening and/or stress responses are indicated.

    Article Snippet: The cDNA synthesis, labelling, hybridization, and washing steps were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide ( https://www.agilent.com/cs/library/usermanuals/Public/G4140-90040_GeneExpression_OneColor_6.9.pdf ).

    Techniques: Expressing, Microarray

    Summary of apoptosis pathways in human and Oncopig cells. Summarized apoptotic pathways altered by gemcitabine treatment in Oncopig BCCL and 5637 cells evaluated by microarray. There were multiple possible cellular pathways involved in apoptosis induction, such as: TP53INP1/P53/BCL/BAX/CASPASE mitochondrial intrinsical pathway; TP53INP1/TP73 activation; FAS/RB1/GADD45B/CASPASE pathway and cell rounding up by RND1 and/or ZMYM6.

    Journal: Frontiers in Oncology

    Article Title: Oncopig bladder cancer cells recapitulate human bladder cancer treatment responses in vitro

    doi: 10.3389/fonc.2024.1323422

    Figure Lengend Snippet: Summary of apoptosis pathways in human and Oncopig cells. Summarized apoptotic pathways altered by gemcitabine treatment in Oncopig BCCL and 5637 cells evaluated by microarray. There were multiple possible cellular pathways involved in apoptosis induction, such as: TP53INP1/P53/BCL/BAX/CASPASE mitochondrial intrinsical pathway; TP53INP1/TP73 activation; FAS/RB1/GADD45B/CASPASE pathway and cell rounding up by RND1 and/or ZMYM6.

    Article Snippet: To obtain the gene transcription panel of treated and untreated human and Oncopig cells, the Two-Color Microarray-Based Gene Expression Analysis microarrays (Agilent Technologies Inc, Santa Clara, CA, USA) were used according to the manufacturer’s instructions.

    Techniques: Microarray, Activation Assay